Yinzhi Lang

Yinzhi Lang, Ph.D.

Research Assistant Professor

Department: Pharmacotherapy & Translational Research
Business Phone: (626) 238-9567
Business Email: y.lang@cop.ufl.edu

About Yinzhi Lang

Yinzhi Lang, Ph.D., joined the UF College of Pharmacy in 2018 and have received systematic academic training on antimicrobial pharmacology, pharmacokinetics and pharmacodynamics. In 2021, she was appointed as a research assistant professor in the college. Dr. Lang created a series of UPLC-MRM-MS/MS assays to quantify the penetration of multiple antibiotic classes and employed LC-MS/MS based proteomics, metabolomics and lipidomics to characterize bacterial biomolecules (porins, efflux pumps, receptors, energy metabolites, lipids). She is significantly contributing to developing Quantitative and Systems Pharmacology (QSP) models with Dr. Bulitta to integrate these mechanistic data with bacterial killing, resistance, tolerance and persistence.

Dr. Lang’s most valuable contribution to the anti-infective research is the series of intracellular penetration assays for multiple antibiotic and anti-viral classes which she developed and/or greatly enhanced. These use different assay approaches to study outer membrane permeability of Gram-negative bacteria as well as direct quantification of intracellular antibiotic or antiviral concentrations (including those of active triphosphate metabolites). Dr. Lang fully leverages our latest generation LC-MS/MS (Sciex 6500+) at UF in Lake Nona. This allows us to move the field forward via translational collaborative research.

Dr. Lang’s doctoral research focused on developing advanced biochemical and bioanalytical methods for extracting and analyzing complex carbohydrate from animals and humans. In her doctoral dissertation, she developed novel combination strategies of carbohydrate derivatization and LC-MS/MS for rapid and reliable compositional profiling of human milk free oligosaccharides (HMOs, ~200 diverse structures). She further developed an integrated design for compositional profiling of the total carbohydrate moiety of human milk, including HMOs, N-/O-linked glycoproteins and glycolipids, and explored the expression dynamics of glycans components associated with different lactation stages, to support future research on optimizing infant formula design. When working as a Senior Scientist at Shanghai Greenvalley Pharmaceutical Co. Ltd., Dr. Lang played an integral role in developing instrumental analysis methods (GC-MS, CE-MS, LC-MS) for QC and ADME studies of an innovative anti-Alzheimer agent GV-971 (Phase III, NCT04520412).

Research Profile

Open Researcher and Contributor ID (ORCID)

0000-0002-6857-4516

Publications

2021
Combating Multidrug-Resistant Bacteria by Integrating a Novel Target Site Penetration and Receptor Binding Assay Platform Into Translational Modeling.
Clinical pharmacology and therapeutics. 109(4):1000-1020 [DOI] 10.1002/cpt.2205. [PMID] 33576025.
2021
Comparison of Different Labeling Techniques for the LC-MS Profiling of Human Milk Oligosaccharides.
Frontiers in chemistry. 9 [DOI] 10.3389/fchem.2021.691299. [PMID] 34589467.
2021
Generating Genotype-Specific Aminoglycoside Combinations with Ceftazidime/Avibactam for KPC-Producing Klebsiella pneumoniae.
Antimicrobial agents and chemotherapy. 65(9) [DOI] 10.1128/AAC.00692-21. [PMID] 34152820.
2020
First Penicillin-Binding Protein Occupancy Patterns for 15 β-Lactams and β-Lactamase Inhibitors in Mycobacterium abscessus.
Antimicrobial agents and chemotherapy. 65(1) [DOI] 10.1128/AAC.01956-20. [PMID] 33106266.
2020
Novel Cassette Assay To Quantify the Outer Membrane Permeability of Five β-Lactams Simultaneously in Carbapenem-Resistant Klebsiella pneumoniae and Enterobacter cloacae.
mBio. 11(1) [DOI] 10.1128/mBio.03189-19. [PMID] 32047131.
2018
Glycosaminoglycanomic profiling of human milk in different stages of lactation by liquid chromatography-tandem mass spectrometry.
Food chemistry. 258:231-236 [DOI] 10.1016/j.foodchem.2018.03.076. [PMID] 29655727.
2018
Separation and Structural Sequence Analysis of Sialylated HMOs via Tandem Mass Spectrometry
Chemical Journal of Chinese Universities. 39(4):645-652 [DOI] 10.7503/cjcu20170593.
2016
In Vivo Anti-Cancer Mechanism of Low-Molecular-Weight Fucosylated Chondroitin Sulfate (LFCS) from Sea Cucumber Cucumaria frondosa.
Molecules (Basel, Switzerland). 21(5) [DOI] 10.3390/molecules21050625. [PMID] 27187337.
2016
Isolation, Purification and Disaccharide Composition Analysis of Chondroitin Sulfate C from IRA Rabbit Lung
Chinese Journal of New Drugs. 25(10):1165-1169
2016
Isolation, purification and structural identification of glycosaminoglycans from porcine lung
Chinese Journal of Pharmaceutical Analysis. 36(4):587-593 [DOI] 10.16155/j.0254-1793.2016.04.05.
2016
Low anticoagulant heparin oligosaccharides as inhibitors of BACE-1, the Alzheimer’s β-secretase.
Carbohydrate polymers. 151:51-59 [DOI] 10.1016/j.carbpol.2016.05.050. [PMID] 27474542.
2015
Extraction, Isolation, Structural Characterization and Anti-Tumor Properties of an Apigalacturonan-Rich Polysaccharide from the Sea Grass Zostera caespitosa Miki.
Marine drugs. 13(6):3710-31 [DOI] 10.3390/md13063710. [PMID] 26110894.
2015
Isolation and Structural Identification of Neutral Human Milk Oligosaccharides
Chemical Journal of Chinese Universities. 36(6):1087-1093 [DOI] 10.7503/cjcu20150118.
2014
Applications of mass spectrometry to structural analysis of marine oligosaccharides.
Marine drugs. 12(7):4005-30 [DOI] 10.3390/md12074005. [PMID] 24983643.
2014
Isolation, purification and fine structure comparison analysis of glycosaminoglycans from three fish gills
Chinese Journal of Marine Drugs. 33(5):1-8 [DOI] 10.13400/j.cnki.cjmd.2014.05.001.
2014
Isolation, purification and physiochemical characteristics comparison study of polysaccharides between wild and low salinity cultured green seaweeds Chaetomorphalinum
Chinese Journal of Marine Drugs. 33(5):31-36 [DOI] 10.13400/j.cnki.cjmd.2014.05.005.
2014
Modification of reduction method about uronic acids in acidic polysaccharides
Chinese Journal of Marine Drugs. 33(4):1-7 [DOI] 10.13400/j.cnki.cjmd.2014.04.001.
2012
Analysis of structural heterogeneity of fucoidan from Hizikia fusiforme by ES-CID-MS/MS.
Carbohydrate polymers. 90(1):602-7 [DOI] 10.1016/j.carbpol.2012.05.084. [PMID] 24751082.

Education

Ph.D.
2016 · Ocean University of China

Contact Details

Phones:
Business:
(626) 238-9567
Emails:
Business:
y.lang@cop.ufl.edu